Further information is available in BGB “Fact Sheet” available at this LINK<\/a><\/span><\/span>.<\/p>\n The most recent scientific evidence is also available at this LINK<\/a><\/span><\/span>; this being an external peer review, published 27 November 2020, undertaken by a highly respected group of 23 international virologists, microbiologists and related scientists. This calls into question the validity of the test for the reasons outlined. This brief article by BGB aims to provide further technical information, specifically regarding the test “Amplification Cycle Threshold” [C t] value.<\/p>\n The following information has been received by BGB on this subject following a request for information directed to Public Health Wales [PHW] and Public Health England [PHE]. The original correspondence is available in BGB’s Correspondence Journal<\/span><\/span><\/a> (volumes 1 & 2)<\/p>\n Confirmation was received in August 2020 that commercial assays used in Wales for the clinical diagnosis of SARS-CoV-2 infection are all CE marked. The number of amplification cycles in tests can vary with different platforms used. Most platforms use threshold cycles that range from 27 to 43. The threshold cycle is determined by the platform used and is not something the laboratory service has control over. Confirmation was also received from PHW in October 2020 that samples from Royal Glamorgan Hospital for Covid testing may be tested in the laboratory in Royal Glamorgan Hospital or laboratories in the Public Health Wales network. The real-time PCR assays in use in Wales for Covid 19 diagnostics all run for 45 cycles<\/span><\/strong>; however, the cycle number where the sample is defined as “RNA not detected” varies by platform and target gene detected by the system. This is defined by the manufacturer. One platform (Hologic) is isothermal, this means it does not cycle through temperature changes in the same way as the real-time PCR systems, therefore CT values are not reported by this system.<\/p>\n Confirmation received 13 November 2020 that PHE does not hold information on testing kits used by non-PHE laboratories. These laboratories have a statutory duty to report “positive cases” to PHE but they are not obliged to advise PHE which tests they are using.<\/p>\n It is important to note that amplification cycles increase exponentially as set out on the table below.<\/p>\n C t<\/b><\/p>\n<\/td>\n Amplification <\/b><\/p>\n<\/td>\n Amplification<\/b><\/p>\n<\/td>\n<\/tr>\n 50<\/p>\n<\/td>\n 1125,899,906,842,624<\/p>\n<\/td>\n 1125 Trillion<\/p>\n<\/td>\n<\/tr>\n 45<\/p>\n<\/td>\n 35,184,372,088,832<\/p>\n<\/td>\n 35,184 Billion<\/p>\n<\/td>\n<\/tr>\n 40<\/p>\n<\/td>\n 1099,511,627,776<\/p>\n<\/td>\n 1099 Billion<\/p>\n<\/td>\n<\/tr>\n 40<\/p>\n<\/td>\n 1099,511,627,776<\/p>\n<\/td>\n 1099 Billion<\/p>\n<\/td>\n<\/tr>\n 35<\/p>\n<\/td>\n 34,359,738,368<\/p>\n<\/td>\n 34 Billion<\/p>\n<\/td>\n<\/tr>\n 30<\/p>\n<\/td>\n 1,073,741,824<\/p>\n<\/td>\n 1 Billion<\/p>\n<\/td>\n<\/tr>\n 20<\/p>\n<\/td>\n 1,048,576<\/p>\n<\/td>\n 1 Million<\/p>\n<\/td>\n<\/tr>\n 15<\/p>\n<\/td>\n 32,768<\/p>\n<\/td>\n 32 Thousand<\/p>\n<\/td>\n<\/tr>\n 10<\/p>\n<\/td>\n 1,024<\/p>\n<\/td>\n 1 Thousand<\/p>\n<\/td>\n<\/tr>\n 5<\/p>\n<\/td>\n 32<\/p>\n<\/td>\n 32<\/p>\n<\/td>\n<\/tr>\n 2<\/p>\n<\/td>\n 4<\/p>\n<\/td>\n 4<\/p>\n<\/td>\n<\/tr>\n A C t threshold value of 45 (recommended rate for of cycles originally recommended by the W.H.O.) amplifies the fragment of RNA by 35,184 billion times<\/span><\/strong>. The test actually measures the presence of partial RNA sequences present in the intact virus, which could be a piece of dead virus which cannot make the subject sick, and cannot be transmitted, and cannot make anyone else sick. A true positive test result does not necessarily indicate the presence of viable virus. In limited studies to date, many researchers have shown that some subjects remain PCR-positive long after the ability to culture virus from swabs has disappeared. They term this a \u2018cold positive\u2019 (to distinguish it from a \u2018hot positive\u2019, someone actually infected with intact virus). The key point about \u2018cold positives\u2019 is that they are not ill, not symptomatic, not going to become symptomatic and, furthermore, are unable to infect others.<\/p>\n The information received from PHW and PHE regarding C t values supports the findings of the external peer review, that the RT-PCR test is not fit for purpose. The Portuguese appeal court, on 11 November 2020, arrived at a similar conclusion. Confirmation from PHW and PHE that kit manufacturers have control over the threshold cycles used in the tests rather than the client; Public Health Authorities, the National Health Service, Welsh Government and UK Government, is a matter of grave concern. It suggests a total lack of control by the client.<\/p>\n I hope this information is helpful to readers. BGB will continue to draw these facts to the attention of decision makers in Government as we have done consistently since the beginning of this ‘pandemic’ scare. See our Correspondence Journal<\/a><\/span><\/span>).<\/p>\n More Information Is Available In This Video:<\/strong><\/p>\nInformation received from Public Health Wales [PHW]<\/h3>\n
Information received from Public Health England [PHE]<\/h3>\n
Comment by BGB<\/h2>\n
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